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12 week-old mice) ( Paracellular transport across the epithelial barrier is regulated by tight junction proteins, which form cell-to-cell complexes and depend on calcium for assembly ( Cldn3, Ocln, Tjp1 (ZO-1), and Jam1 in the small intestine is lower in newborns (1 day and 2-week-old mice), while the expression of Cldn2 is higher compared to adults (12-week-old mice) ( Intestinal alkaline phosphates is a brush border enzyme expressed and secreted by enterocytes ( in vit ro ( in vivo (24)