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Our initial hypothesis was that MDD is linked to a reduction in the secretion and production of critical cytokines that influence the immune system response, including IL-1, IL-6, IL-2, and interferon-gamma (IFN-)

Briefly, the yeast cell pellet was collected by centrifugation in a microcentrifuge tube and washed three times with sterile water, then quenched with pre-chilled 80% methanol (kept at 80C), followed by seven cycles of bead beating (using glass beads)