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It is suggested that moderate precursor supplementation and normal mitochondrial function are important prerequisites for maintaining the effective action of NAD+ protection signaling on aging cardiomyocytes

Examples of detectable substances include various enzymes, prosthetic groups, fluorescent materials, luminescent materials, bioluminescent materials, radioactive materials, positron emitting metals using various positron emission tomographies, and nonradioactive paramagnetic metal ions

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Monari et al., 1999)