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2.6 In vivo Therapeutic Efficacy Assessment on DED 2.6.1 Animal Models of DED All experimental procedures for animal experiments were performed in accordance with the guidelines of the Chinese Animal Administration and the guidelines of the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research

Supernatants were isolated to quantify protein and 510 g protein samples were mixed with a 4X non-reducing buffer prior to separation by SDS-PAGE as described