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After several washes in TBS-T, membranes were incubated in a 1:30:000 dilution of IRDye 800 donkey anti-rabbit or anti-mouse (Licor, Lincoln, NE) in 5% dry non-fat milk powder and 0.02% SDS in TBS-T for 1 hour at room temperature

Water could then leak into an empty pocket behind the ear drum, creating an ideal environment for bacteria to grow and cause infection