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Thymosin Alpha-1 Thymosin Alpha-1 modulates immune function in ways that support tissue regeneration indirectly

After application of an ice pack to the lower lid and cheek, a local anesthetic consisting of 0.5% lidocaine with epinephrine (0.2 to 0.4 mL) is injected into the orbicularis within the boundaries of the tear trough

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Following, the cells were washed two times by centrifugation in PBS (450g for 5 min)