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The optical density (OD) at 490 nm was recorded as an estimate of remaining viable cell number by making use of a microtiter plate reader (BioTek Instruments)

In addition, push out a tiny amount of solution to ensure there is no air left in the metal tip

Concentration = vial mcg / water mL Dose volume = target dose mcg / concentration U-100 units = dose volume mL x 100 Semaglutide 5 mg vial with 2 mL example A 5 mg semaglutide vial mixed with 2 mL gives a concentration of 2.5 mg/mL, or 2,500 mcg/mL