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SOCE were triggered by depleting Ca 2+ from the ER with cyclopiazonic acid (10 M, CPA), a reversible SERCA (Sarcoplasmic and Endoplasmic Reticulum Ca 2+ ATPase) pump blocker, in Ca 2+ -free medium, and revealed by reinstating extracellular calcium concentration at 2 mM (Figure 2C)

Clinically, there are distinctions between these two conditions, such as Stemmers sign, which is considered positive in lymphedema, when the skin at the base of the second toe cannot be pinched and lifted due to skin and underlying tissue thickening

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