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These metabolites exhibited significant inhibition of nitric oxide production in lipopolysaccharide-stimulated BV-2 microglial cells, with EC 50 values ranging from 2.32 to 23.83 M

G1E-ER4 cells were cultured in Iscoves MDM medium (Life Technologies, #12440-061) supplemented with 15% heat-inactivated foetal calf serum (Interpath, #SFBSF13), 1% PSG (Life Technologies, #10378016), 62 l of 10% monothioglycerol (Sigma-Aldrich, #M6145), 0.6% home-made Kit-ligand conditioned medium, 50ul mouse erythropoietin (Biolegend, #587606) in 500 ml IMDM medium 39

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Arteriosclerosis and atherosclerosis assessment in clinical practice: methods and significance