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RSL3-NH2 hydrochloride exhibits significant cytotoxicity against colorectal cancer cells and effectively induces their ferroptosis

In brief, 50-100 mg leaf samples were frozen in liquid nitrogen, and ground with 1 mL Extraction Buffer [50 mM EPPS buffer, pH 8.0, containing 1 mM EDTA, 10 mM DTT, 0.1% Triton X-100 [v/v], 0.5% polyvinylpyrrolidone, and 1x SigmaFAST Protease Inhibitor Cocktail, EDTA Free (Sigma)] on ice using 2 mL glass-to-glass homogenizer

Type C phospholipase A subclass of enzymes that cleave the polar head group of phosphoinositides between the glycerol and phosphate moieties

Small amounts of unmetabolized quercetin are metabolized in the hepatic and intestinal circuits and enter the systemic circulation or are excreted in the bile