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1) is distinct among sirtuins due to its ability to remove specific acyl post-translational modifications (PTMs), including succinylation, malonylation, and glutarylation, thereby modulating the function of both mitochondrial and cytoplasmic proteins

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2 Assay principle Cells expressing G protein-gated inwardly rectifying potassium channels (GIRK) were loaded with thallium as a surrogate for potassium and Thallos, a thallium-sensitive dye (Figure 1)