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six animals from the LD control, WD control, and WD/LD-V groups and five animals from the WD/LD-T group were euthanized the first day

The current paradigm is that compromised V-ATPase function impairs starvation-induced autophagy 16 , because most lysosomal/vacuolar hydrolytic enzymes have optimal activity at acidic pH 17

Everts B, Amiel E, Huang SC, Smith AM, Chang CH, Lam WY, Redmann V, Freitas TC, Blagih J, van der Windt GJ, et al

it is also a major NAD + consumer with a lower K m than most NAD + -consuming enzymes 40 and is implicated in the decline of NAD + levels observed during aging and disease 5052