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To each sample, 9 l of HPLC-grade acetonitrile was added followed by 3 l (20 g l 1 in dry acetonitrile) of the respective 6plex tandem mass tag (TMT) and the reaction was vortexed and incubated at room temperature for 1 h with additional vortexing every 20 min

Alkaloids are ubiquitous in nature, especially in the plant kingdom

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