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Plasma was rapidly separated from cellular components by centrifuging at 3300 g at 4C for 15 min

Across these trials, tesamorelin at 2 mg subcutaneously once daily produced a statistically significant reduction in visceral adipose tissue and waist circumference over 26 weeks, with 26-week extension phases showing that the visceral-fat improvement was maintained on continued treatment and, importantly, without adverse effects on blood glucose or lipid parameters in those analyses

For hPrdx6 protein purification, the crude protein extract was passed through the Ni-NTA agarose column pre-equilibrated with binding buffer (20 mM Tris (pH 7.0), 50 mM NaCl, 10 mM Imidazole), followed by washing and elution using wash buffer (20 mM Tris (pH 7.0), 50 mM NaCl, 60 mM Imidazole) and elution buffer (20 mM Tris (pH 7.0), 50 mM NaCl, 500 mM Imidazole), respectively

Sesti G, Sciacqua A, Cardellini M, Marini MA, Maio R, Vatrano M, Succurro E, Lauro R, Federici M, Perticone F: Plasma concentration of IGF-I is independently associated with insulin sensitivity in subjects with different degrees of glucose tolerance